嚴冬

發布時間：2021-07-12瀏覽次數：5925

教師基本信息

姓名：嚴冬

職稱：研究員

電子郵箱：yandong@fudan.edu.cn

辦公地點：万博英超狼队网官方网 A501-5室

辦公電話：021-31246583


研究方向

RNA修飾介導的發育與代謝研究

RNA中有超過100種化學修飾被發現，它們的生物學功能和調控機製才剛剛開始被揭示，因此這些科學問題成為目前國際研究的焦點並形成了一個新的領域，表觀轉錄組學(epitranscriptomics)。我們前期首次鑒定了N6-甲基腺嘌呤(m6A)甲基轉移酶複合物(又稱為writer complex)三個新的、在進化中保守的組分Nito/Rbm15、Xio/ZC3H13和Hakai，並發現它們通過修飾果蠅性別決定主導基因Sxl的mRNA，從而調控其可變剪接(alternative splicing)來控製果蠅的性別決定。實驗室今後將使用果蠅和小鼠模型在以下幾個方麵開展研究：(1)篩選m6A通路中新的因子及動物性別決定途徑新的成員；(2)解析m6A修飾在發育和代謝過程中的作用機理；(3)構建在動物體內修改RNA修飾的體係；(4)利用已構建的m6A報告係統篩選影響這一過程的化學小分子；(5)利用在m6A研究過程中積累的技術和手段展開對其它RNA修飾的功能性探索。


個人簡介

2000年獲南京大學理學學士；2009年獲美國辛辛那提大學分子與發育生物學博士；2010-2016年在美國哈佛大學醫學院遺傳學係，Howard Hughes Medical Institute，從事博士後研究；2016-2020年在中科院上海植物生理生態研究所/分子植物卓越中心任研究員及課題組長；2021年任万博英超狼队网官方网 研究員。入選國家青年拔尖人才、上海市海外高層次人才和浦江人才計劃。在Developmental Cell, PNAS, Nature Communications等國際學術期刊發表論文26篇；任《遺傳》期刊編委。目前承擔國家自然科學基金麵上項目、重大研究計劃、科技部重點研發計劃等項目。


獲獎情況

2019，國家青年拔尖人才

2019，上海市海外高層次人才

2017，上海市浦江人才計劃

2014，Charles A. King Trust博士後獎學金

2012，Damon Runyon Cancer Research Fellowship

2005，Albert J. Ryan Fellowship

2005，美國心髒協會(AHA)博士獎學金


授課情況

《發育與代謝》


招生專業

細胞與發育生物學


代表性論文和論著

  1.  Wang, Y., Zhang, L., Ren, H., Ma, L., Guo, J., Mao, D., Lu, Z., Lu, L. and Yan, D.* (2021) Role of Hakai in m6A modification pathway in Drosophila. Nature Communications, 12(1):2159.

  2. Zirin, J., Hu, Y., Liu, L., Yang-Zhou, D., Colbeth, R., Yan, D., Ewen-Campen, B., Tao, R., Vogt, E., VanNest, S., Cavers, C., Villalta, C., Comjean, A., Sun, J., Wang, X., Jia, Y., Zhu, R., Peng, P., Yu, J., Shen, D., Qiu, Y., Ayisi, L., Ragoowansi, H., Fenton, E., Efrem, S., Parks, A., Saito, K., Kondo, S., Perkins, L., Mohr, S., Ni, J. and Perrimon, N. (2020) Large-Scale Transgenic Drosophila Resource Collections for Loss- And Gain-of-Function Studies. Genetics, 214(4):755-767.

  3. Silver, J., Wirtz-Peitz, F., Simoes, S., Pellikka, M., Yan, D., Binari, R., Nishimura, T., Li, Y., Harris, T., Perrimon, N. and Tepass, U. (2019) Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly. The Journal of Cell Biology, 218(10), 3397-3414.

  4. Guo, J., Tang, H., Li, J., Perrimon, N.* and Yan, D.* (2018) Xio is a novel component of the Drosophila sex determination pathway and RNA N6-methyladenosine methyltransferase complex. PNAS, 115(14):3674-3679.

  5. Li, Z., You, L., Yan, D., James, A. Huang, Y. and Tan A. (2018) Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination. PLoS Genetics, 14(2), e1007245.

  6. Kan, L., Grozhik, A., Vedanayagam, J., Patil, D., Pang, N., Lim, K., Huang, Y., Joseph, B., Lin, C., Despic, V., Guo, J., Yan, D., Kondo, S., Deng, W., Dedon, P., Jaffrey, S. and Lai, E. (2017) The m6A pathway facilitates sex determination in Drosophila. Nature Communications, 8:15737.

  7. Yan, D. and Perrimon, N. (2015) Spenito is required for sex determination in Drosophila melangaster. PNAS, 112(37):11606-11611.

  8. Sun, J., Wei, H., Xu, J., Chang, J., Yang, Z., Ren, X., Lv, W., Liu, L., Pan, L., Wang, X., Qiao, H., Zhu, B., Ji, J., Yan, D., Xie, T., Sun, F. and Ni, J. (2015) Histone H1-Mediated Epigenetic Regulation Controls Germline Stem Cell Self-Renewal by Modulating the Acetylation of H4 Lysine 16. Nature Communications, 6:8856.

  9. Perkins, L., Holderbaum, L., Tao, R., Hu, Y., Sopko, R., McCall, K., Yang-Zhou, D., Flockhart, I., Binari, R., Shim, H., Miller, A., Housden, A., Foos, M., Randklev, S., Kelley, C., Namgyal, P., Villalta, C., Liu, L., Jiang, X., Huan-Huan, Q., Xia, W., Fujiyama, A., Toyoda, A, Ayers, K., Blum, A., Czech, B., Neumuller, R., Yan, D., Cavallaro, A., Hibbard, K., Hall, D., Cooley, L., Hannon, G., Lehmann, R., Parks, A., Mohr, S., Ueda, R., Kondo, S., Ni, J. and Perrimon, N. (2015) The Transgenic RNAi Project at Harvard Medical School: Resources and Validation. Genetics, 201(3):843-52.

  10. Zeng, X., Han, L., Singh S., Liu, H., Neumüller, R., Yan, D., Hu, Y., Liu, Y., Liu W., Lin, X. and Hou, S. (2015) Genome-Wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila. Cell Reports, 10(7): 1226-1238.

  11. Droujinine, I., Yan, D. and Perrimon, N. (2014) A sharp end to sugary Wingless travels. The Journal of Cell Biology. 206(7):819-821(Comment).

  12. Yan, D., Neumuller, R., Buckner, M., Ayers, K., Li, H., Hu, Y., Yang-Zhou, D., Pan, L., Wang, X., Kelley, C., Vinayagam, A., Binari, R., Randklev, S., Perkins, L., Xie, T., Cooley, L. and Perrimon, N. (2014) A regulatory network of Drosophila germline stem cell self-renewal. Developmental Cell, 28(4): 459-473.

  13. Staller, M., Yan, D., Randklev, S., Bragdon, M., Wunderlich, Z., Tao, R., Perkins, L., DePace, A. and Perrimon, N. (2013) Depleting Gene Activities in Early Drosophila Embryos with the “Maternal-Gal4–shRNA” System. Genetics, 193(1): 51-61.

  14. Friedman, A., Tucker, G., Singh, R., Yan, D., Arunachalam, V., Hu, Y., Binari, R., Hong, P., Sun, X., Porto, M., Pacifico, S., Murali, T., Finley, R., Asara, J., Berger, B. and Perrimon, N. (2011) Proteomic and functional genomic landscape of receptor tyrosine kinase and Ras/ERK signaling. Science Signaling, 4, rs10.

  15. Yan, D., Wu, Y., Yang, Y., Belenkaya, T., Tang, X. and Lin, X. (2010) The cell-surface proteins Dally-like and Ihog differentially regulate Hh signaling strength and range during development.Development, 137, 2033-2044.

  16. Yan, D., Wu, Y., Feng, Y., Lin, S. and Lin, X. (2009) The core protein of glypican Dally-like determines its biphasic activity in Wingless morphogen signaling. Developmental Cell, 17(4): 470-481.

  17. Yan, D and Lin, X. (2009) Shaping morphogen gradient by proteoglycans. In “Generation and Interpretation of Morphogen Gradients”, Cold Spring Harbor Laboratory Press (book chapter).

  18. Yan, D and Lin, X. (2008) Opposing roles for glypicans in Hedgehog signalling. Nature Cell Biology, 10(7):761-3 (news and views).

  19. Belenkaya, T., Wu, Y., Tang, X., Zhou, B., Cheng, L., Sharma, Y., Yan, D.,Selva, E. and Lin, X. (2008) The Retromer Complex Influences Wnt Secretion by Recycling Wntless from Endosomes to the Trans-Golgi Network. Developmental Cell, 14(1): 120-131.

  20. Yan, D. and Lin, X.(2007)Drosophila glypican Dally-like acts in FGF-receiving cells to modulate FGF signaling during tracheal morphogenesis.Developmental Biology, 312(1):203-16.

  21. Han, C. #, Yan, D. #, Belenkaya, T. and Lin, X.(2005) Drosophila Glypicans Dally and Dally-like shape the extracellular Wingless morphogen gradient in the wing disc. Development, 132, 667-679.

  22. Tao, Q., Yokota, C., Puck, H., Kofron, M., Birsoy, B., Yan, D., Asashima, M., Wylie.C.C, Lin, X. and Heasman, J. (2005) Maternal Wnt11 activates the canonical Wnt signaling pathway required for axis formation in Xenopus embryos. Cell, 120(6): 857-871.

  23. Belenkaya, T., Han, C., Yan, D., Opoka, R.J., Khodoun, M., Liu, H. and Lin, X. (2004) Drosophila Dpp morphogen movement is independent of dynamin-mediated endocytosis and is controlled by glypican members of heparan sulfate proteoglycans. Cell, 119(2): 231-44.

  24. Yang, L., Yan, D., Bruggeman, M., Du, H. and Yan, C. (2004) Mutation of a lysine residue in a homeodomain generates dominant negative thyroid transcription factor 1. Biochemistry, 43(39), 12489-97.

  25. Yang, L., Yan, D., Yan, C. and Du, H. (2003)Peroxisome proliferator-activated receptor gamma and ligands inhibit surfactant protein B gene expression in the lung. The Journal of Biological Chemistry, 278, 36841-36847.

  26. Yang, L., Naltner, A., Kreiner, A., Yan, D., Cowen, A., Du, H. and Yan, C. (2003) An enhancer region determines hSP-B gene expression in bronchiolar and ATII epithelial cells in transgenic mice. American Journal of Physiology Lung Cellular and Molecular Physiology, 284(3): L481-8.


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