教師基本信息/Basic Information
姓名/Name：市川宗嚴/ICHIKAWA Muneyoshi
職稱/Title：青年研究員/Tenure-track professor
職務/Position：研究組長/PI
電子郵箱/Email address：ichikawa_muneyoshi@fudan.edu.cn
辦公地點/Address：
上海市楊浦區淞滬路2005號万博体育分 A201-5
Room A201-5, School of Life Sciences, 2005 hao, Songhu-lu, Yangpu-qu, Shanghai 200438, China
個人簡介/Education and Research Background
市川宗嚴，於2010年獲得日本東京大學學士學位。研究生期間，在東京大學豐島陽子(TOYOSHIMA Yoko)教授的指導下針對動力蛋白（dynein）的結構和功能開展研究，入選日本學術振興會（JSPS）特別研究員（DC1）後，於2015年獲博士學位。作為博士後加入加拿大麥吉爾大學Khanh-Huy Bui課題組工作後，通過冷凍電子顯微鏡研究纖毛的雙微管結構，並入選日本學術振興會（JSPS）海外特別研究員。2019年，任日本奈良先端科學技術大學院大學助理教授（塚崎智也教授課題組），從事X射線晶體學研究。2020年入選日本科學技術振興機構（JST）PRESTO研究員，致力於新型人工微管組裝技術的研發。於2022年8月加入万博英超狼队网官方网 。
Muneyoshi ICHIKAWA received his bachelor’s degree from The University of Tokyo in 2010. He did his master and doctor course with Prof. Yoko Y. Toyoshima at Graduate School of Asts and Sciences, The University of Tokyo. During this time, he studied the structure and function of motor protein dynein. He was also chosen as a JSPS research fellow (DC1). He was granted his doctor degree in 2015. He then joined Bui lab from McGill University, Canada as a postdoctoral fellow and JSPS overseas research fellow, where he worked on structure of doublet microtubule from cilia by cryo-EM. After that, he did Assistant Professor at Prof. Tomoya Tsukazaki’s lab at Nara Institute of Science and Technology and worked on X-ray crystallography. He was selected as a JST PRESTO researcher from 2020. He also generated a novel microtubule manipulation technique. He joined School of Life Sciences, Fudan University in August 2022.
研究方向/Research Direction
研究方向1:纖毛蛋白複合體的結構解析
纖毛是附著在真核細胞表麵的微小毛發狀結構，是一個複雜的9+2結構，由600多種蛋白質組成。纖毛不但對於推動精子等細胞運動非常重要，同時還接收來自外環境的各種信號。因此，纖毛蛋白的缺陷與許多人類疾病有關(纖毛病)。為了了解纖毛的功能和開發新的纖毛病的治療方法，獲取纖毛蛋白複合體乃至整體纖毛的三維結構是至關重要的。
Research Direction1: Structural analysis of ciliary protein complexes.Cilia are tiny hair like structures attached to eukaryotic cell surfaces. Cilia are important for propelling motility of the cells like sperm cells. Cilia also receive signals from outside environment. Thus, defects of ciliary proteins are linked to many human diseases collectively termed as ciliopathy. Cilia have a complex 9+2 architecture and composed of over 600 kinds of proteins. To understand how cilia function and to develop new treatment for ciliopathy, it is crucial to obtain structures of ciliary protein complexes and whole ciliary structure.
研究方向2: 人工微管組裝技術——研發操縱微管結構和運動蛋白的新方法在真核細胞中，微管就像是一條鐵路，運動蛋白（如驅動蛋白kinesin和動力蛋白dynein）在微管上行走，運輸貨物。通過了解微管和運動蛋白的結構和功能，我們可以對這些蛋白進行突變，以實現在材料科學和納米技術上的新應用。
Research Direction2: Generating novel method to manipulate microtubule structures and motor proteins. In the eukaryotic cells, microtubules serve as a railway and motor proteins, such as kinesins and dyneins walk on microtubules to transport cargoes. By understanding the structures and functions of microtubules and motor proteins, we can genetically engineer these proteins for novel application for material science and nanotechnology.
生專業/Majors
生物物理和結構生物學/Biophysics and structural biology
獲獎情況/Awards and Honors
・2018, 日本生物物理學會青年獎勵獎/Early Career Award in Biophysics
・2017, Gérard T. Simon博士獎/Dr. Gérard T. Simon Award
・2016, 青年優秀發表獎/Young Scientist Award
代表性論文和論著/Selected Publications
Inaba, H.†*, Sueki, Y.†, Ichikawa, M.†, Kabir, A.M.R., Iwasaki, T., Shigematsu, H., Kakugo, A., Sada, K., Tsukazaki, T. & Matsuura, K.*
Science Advances, in press[†共同第一作者, equally contributed]
Kubo, S.†, Yang, S. K.†, Black, C., Dai, D., Valente, M., Gaertig, J., Ichikawa, M.*, & Bui, K. H.*
Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM.
EMBO Reports, 22(9): e52911(2021) [*共同通訊作者, co-corresponding authors]
Black, C.†, Dai, D.C.†, Peri, K. Ichikawa, M.*, & Bui, K. H.*
Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy.
Bio-protocol, 11(11), e4041(2021) [*共同通訊作者, co-corresponding authors]
Dai, D., Ichikawa, M.*, Peri, K., Rebinsky, R., & Bui, K.H.*
Identification and mapping of central pair proteins by proteomic analysis.
Biophysics and Physicobiology, 17, 71-85 (2020)[*共同通訊作者, co-corresponding authors]
Khalifa, A.A.Z.†, Ichikawa, M.†, Dai, D., Kubo, S., Black, C.S., Peri, K., McAlear, T.S., Veyron, S.,Yang, S.K., Vargas, J., Bechstedt, S., Trempe, J.F., & Bui, K.H.* The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications.
eLife, 9, e52760 (2020) [†共同第一作者, equally contributed]
Ichikawa, M., Khalifa, A.A.Z., Kubo, S., Dai, D., Basu, K., Maghrebi, M.A.F., Vargas, J., & Bui, K.H.*Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins.
Proc. Natl. Acad. Sci. USA, 116(40), 19930-19938 (2019) [第一作者, first author]
Ichikawa, M., Liu, D., Kastritis, P.L., Basu, K., Hsu, T.Z., Yang, S., & Bui, K.H.* Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins. Nature Communications, 8, 15035 (2017)[第一作者, first author]
Ichikawa, M., Saito, K., Yanagisawa, H., Yagi, T., Kamiya, R., Yamaguchi, S., Yajima, J., Kushida, Y.,Nakano, K., Numata, O., & Toyoshima, Y.Y.*
Axonemal dynein light chain-1 locates at the microtubule-binding domain of the γ heavy chain. Molecular Biology of the Cell, 26(23), 4236-4247 (2015)[第一作者, first author] [獲F1000Prime推薦, Recommended inF1000Prime]
Torisawa, T., Ichikawa, M., Furuta, A., Saito, K., Oiwa, K., Kojima, H., Toyoshima, Y.Y.*, & Furuta,K.* Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein.
Nature CellBiology,16(11), 1118-1124 (2014)