姓名:甘建華(Jianhua Gan)
職稱:研究員(Professor)
電子郵箱:ganjhh@fudan.edu.cn
辦公地點:生科院A201-7(Building of Life Sciences, Rm A201-7)
辦公電話:021-31246543
研究方向
以X-射線晶體學為主要手段,結合其他生物物理和生物化學方法研究生物大分子的結構和功能。主要包括:(1)病原體關鍵蛋白的結構與功能研究;(2)DNA複製和修複酶的結構和作用機製研究;(3)具有催化功能的核酸分子的結構研究。
We mainly focus on the structural and functional studies of important macromolecules, including (1) pathogenesis-related proteins, (1) proteins involved in DNA replication and DNA repair pathways, and (3) nucleic acids with catalytic activities.
個人簡介
研究員,博導。1997年獲北京醫科大學藥物化學學士學位。同年進入中國科學院上海有機化學研究所,師從中國早期著名的晶體學家夏宗薌教授,從事蛋白質晶體學方麵的研究。2002年獲有機所化學博士學位。在2003-2012年間先後以Postdoctoral Fellow,Research Associate 和Research Scientist 身份在美國國立衛生院(NIH)下屬的癌症研究中心(NCI),美國紐約紀念斯隆凱特琳癌症中心及美國佐治亞州立大學進行研究。2006年榮獲USA The SER-CAT Young Investigator Award (the Southeast Regional Collaborative Access Team,SER-CAT);2007年獲得USA FARE Award (The Fellows Award for Research Excellence, NIH); 2010-2011年獲得USA STTR Grant基金資助。2012年9月受聘狗万外围充值 生命科學學院研究員。
EMPLOYMENT HISTORY
09/2012-present: Professor, School of Life Sciences, Fudan University,
Shanghai
06/2009-08/2012: Research Scientist, Georgia State University, GA
04/2008-04/2009: Research Associate, Memorial Sloan-Kettering Cancer
Center, NY
05/2003-04/2008: Postdoctoral Fellow, NCI, National Institutes of Health,
Frederick, MD
08/2002-05/2003: Research Associate, Shanghai Institute of Organic
Chemistry, Chinese Academy of Sciences, Shanghai
EDUCATION
09/1997-07/2002: Ph.D in Organic Chemistry, Shanghai Institute of Organic
Chemistry, Chinese Academy of Sciences, Shanghai
09/1993-07/1997: B.S. in Department of Pharmaceutical Chemistry, Beijing Medical University, Beijing
PROFESSIONAL ACTIVITY
Judge for the FARE 2008 Travel Award Competition, National Institutes of Health, Bethesda, USA (June, 2007)
AWARDS AND HONORS
STTR Research Award (2011)
The Fellows Award for Research Excellence (FARE), NIH, Bethesda, USA (2007)
The Spring Research Festival Poster Award, NCI-Fort Detrick, Frederick, USA (2007)
The SER-CAT Young Investigator Award, the Southeast Regional Collaborative Access Team (SER- CAT), USA (2006)
Exceptional Pay Increase, National Cancer Institute, NIH, USA (2006)
The Excellent Graduate Award, Beijing Medical University, Beijing, China (1997)
Peking Honor Graduate Award, Beijing, China (1997)
授課情況:生物化學榮譽課程(Biochemistry Honor Program)、結構生物學(Structural Biology)
招生專業:生物化學(Biochemistry)
科研項目:國家自然科學基金麵上項目、科技部重點研發項目
代表性論文和論著:
Z. Shao, S. Su, J. Yang, W. Zhang, Y. Gao, X. Zhao, Y. Zhang, Q. Shao, C. Cao, H. Li, H. Liu, J. Zhang, J. Ma, J. Gan*, “Structures and implications of the C962R protein of African swine fever virus”Nucleic Acids Res, 51(17): 9475-9490 (2023)
H. Liu, Y. Gao, J. Mathivanan, Z. Armour-Garb, Z. Shao, Y. Zhang, X. Zhao, Q. Shao, W. Zhang, J. Yang, C. Cao, H. Li, J. Sheng*, J. Gan*, “Crystal structures and identification of novel Cd2+-specific DNA aptamer”Nucleic Acids Res, 51(9): 4625-4636 (2023)
Z. Shao, J. Yang, Y. Gao, Y. Zhang, X. Zhao, Q. Shao, W. Zhang, C. Cao, H. Liu, J. Gan*, “Structural and functional studies of PCNA from African swine fever virus.” J. Virol, 97(8): e0074823 (2023)
Y. Li, S. Su,Y. Gao, G. Lu, H. Liu, X. Chen, Z. Shao, Y. Zhang, Q. Shao, X. Zhao, J. Yang, C. Cao, J. Lin, J. Ma, J. Gan*,“Crystal structures and insights into precursor tRNA 5'-end processing by prokaryotic minimal protein-only RNase P” Nat Commun. 13(1):2290 (2022)
Y. Zhang, Z. Shao, Y. Gao, B. Fan, J. Yang, X. Chen, X. Zhao, Q. Shao, W. Zhang, C. Cao, H. Liu, J. Gan*,“Structures and implications of the nuclease domain of human parvovirus B19 NS1 protein”, Comput Struct Biotec J, 20: 4645-4655 (2022)
B. Wang, Q. Luo, Y. Li, K. Du, Z. Wu, T. Li, W. Shen, C. Huang*, J. Gan*, A. Dong*, “Structural insights into partner selection for MYB and bHLH transcription factor complexes” Nat Plants, 8(9): 1108-1117 (2022)
R. Cui, H. Li, J. Zhao, X. Li, J. Gan*, J. Ma*, “Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1”Nucleic Acids Res, 50(17): 10169-10186 (2022)
Y. Xu, J. Yang, W. Li, S. Song, Y. S, L. Wu, J. Sun, Me. Hou, J. Wang, X. Jia, H. Zhang, M. Huang, T. Lu, J. Gan*, Y. Feng*, “Three enigmatic BioH isoenzymes are programmed in the early stage of mycobacterial biotin synthesis, an attractive anti-TB drug target” Plos Pathog, 18(7): e1010615 (2022)
G. Cui, Y. Zhang, X. Xu, Y. Liu, Z. Li, M. Wu, J. Liu*, J. Gan*, H. Liang*, “PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa” Sci Adv, 8(49): eadd4220 (2022)
Y. Gao, H. Liu, C. Zhang, S. Su, Y. Chen, X. Chen, Y. Li, Z. Shao, Y. Zhang, Q. Shao, J. Li, Z. Huang, J. Ma, J. Gan*, “Structural basis for guide RNA trimming by RNase D ribonuclease in Trypanosoma brucei”, Nucleic Acids Res. 49(1): 568-583 (2021)
H. Jiang, Y. Gao, L. Zhang, D. Chen*, J. Gan*, A. Murchie*,“The identification and characterization of a selected SAM-dependent methyltransferase ribozyme that is present in natural sequences” Nat Catal, 4: 872-881 (2021)
X. Chen, S. Su, Y. Chen, Y. Gao, Y. Li, Z. Shao, Y. Zhang, Q. Shao, H. Liu, J. Li, J. Ma, J. Gan*, “Structural studies reveal a ring-shaped architecture of deep-sea vent”, Nucleic Acids Res. 48(6): 3343-3355 (2020)
Y. Chen, X. Chen, Q. Huang, Z. Shao, Y. Gao, Y. Li, C. Yang, H. Liu, J. Li, Q. Wang, J. Ma, Y. Zhang*, Y, Gu*, J. Gan*, “A unique DNA-binding mode of African Swine fever virus AP endonuclease”, Cell Discov, 6:13 (2020)
Zhang, H. Zhang, X. Xu, Y. Wang, W. Chen, Y. Wang, Z. Wu, N. Tang, Y. Wang, S. Zhao, J. Gan*, Q. Ji*, “Catalytic-state structure and engineering of Streptococcus thermophiles Cas9”, Nat Catal, 3: 813-823 (2020)
B. Wang, Q. Luo,Y. Li, L. Yin, N. Zhou, X Li, J. Gan*, A. Dong*, “Structural insights into target DNA recognition by R2R3-MYB transcription factors” Nucleic Acids Res. 48 (1): 460-471 (2020)
Y. Chen, H. Liu, C. Yang, Y. Gao, X. Yu, X. Chen, R. Cui, L. Zheng, S. Li, X. Li, J. Ma, Z. Huang*, J. Li*, J. Gan*, “Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues”, Nature Commun. 10(1):387 (2019)
Qian, H. Yu, P. Li, E. Zhu, Q. Yao, C. Tai, Z. Deng, K. Gerdes, X. He*, J. Gan*, H. Ou*, “Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex”, Nucleic Acids Res. 47(14): 7690-7702 (2019)
W. Chen, H. Zhang, Y. Zhang, Y. Wang, J. Gan*, Q. Ji*, “Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease” PloS Biol 17(10):e3000496 (2019)
H. Liu, R. Wang, X. Yu, F. Shen, W. Lan, P. Haruehanroengra, Q. Yao, J. Zhang, Y. Chen, S. Li, B. Wu, L. Zheng, J. Ma, J. Lin, C. Cao*, J. Li*, J. Sheng*, J. Gan*, “High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads”, Nucleic Acids Res. 46(21): 11627-11638 (2018)
C. Yang, R. Wu, H. Liu, Y. Chen, Y. Gao, X. Chen, Y. Li, J. Ma, J. Li*, J. Gan*, “Structural insights into DNA degradation by human mitochondrial nuclease MGME1”, Nucleic Acids Res. 46(20): 11075-11088 (2018)
H. Liu#, X. Yu#, Y. Chen, J. Zhang, B. Wu, L. Zheng, P. Haruehanroengra, R. Wang, S. Li, J. Lin, J. Li, J. Sheng, Z. Huang*, J. Ma*, J. Gan*, “Crystal structure of an RNA-cleaving DNAzyme”, Nat Commun. 8(1): 2006 (2017)
H. Liu, F. Shen, P. Haruehanroengra, Q. Yao, Y. Cheng, Y. Chen, C. Yang, J. Zhang, B. Wu, Q. Luo, R. Cui, J. Li, J. Ma*, J. Sheng*, J. Gan*, “A DNA structure containing AgI-mediated G:G and C:C base pairs”, Angew Chem Int Ed Engl. 56(32):9430-9434 (2017)
H. Liu#, C. Chen#, P. Haruehanroengra, Q. Yao, Y. Chen, C. Yang, Q. Luo, B. Wu, J. Li, J. Ma*, J. Sheng*, J. Gan*, “Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex”, Nucleic Acids Res, 45(5):2910-2918 (2017)
F. Shen, Z. Luo, H. Liu, R. Wang, S. Zhang, J. Gan*, J. Sheng*, “Structural insights into RNA duplexes with multipile 2’-5’-linkages”, Nucleic Acids Res. 45(6):3537-3546 (2017)
Y. Chen, J. Zhang, H. Liu, Y. Gao, X. Li, L. Zheng, R. Cui, Q. Yao, L. Rong, J. Li, Z. Huang*, J. Ma*, J. Gan*, “Unique 5’-P recognition and basis for dG:dGTP misincorporation of ASFV DNA polymerase X”, PloS Biol 15(2):e1002599 (2017)
J. Zhang, H. Liu, Q. Yao, X. Yu, Y. Chen, R. Cui, B. Wu, L. Zheng, J. Zuo, Z. Huang*, J. Ma*, J. Gan*, “Structural basis for single-stranded RNA recognition and cleavage by C3PO”, Nucleic Acids Res, 44(19):9494-9504 (2016)
J. Zhao#, X. Yu#, M. Zhu, H. Kang, J. Ma, M. Wu, J. Gan*, X. Deng*, H. Liang*, Structural and molecular mechanism of CdpR involved in quorum-sensing and bacterial virulence in Pseudomonas aeruginosa, PloS Biol, 14(4):e1002449 (2016).
J. Gan, J. Tropea, B. Austin, D. Court, D. Waugh, X. Ji*, “Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III” Cell, 124(2): 355–366 (2006)