姓名：甘建華
職稱：研究員
電子郵箱：ganjhh@fudan.edu.cn
辦公地點：生科院A201-7
辦公電話：021-31246543
個人網頁/課題組主頁：
研究方向
以X-射線晶體學為主要手段，結合其他生物物理和生物化學方法研究生物大分子的結構和功能。主要包括：（1）RNA沉默通路中的核糖核酸酶的作用機製研究；（2）DNA複製和修複酶的結構和作用機製研究；（3）具有催化功能的核酸分子的結構研究。
個人簡介
（教育工作經曆，學術兼職，科研項目，獲獎情況等）研究員，博導。1997年獲北京醫科大學藥物化學學士學位。同年進入中國科學院上海有機化學研究所，師從中國早期著名的晶體學家夏宗薌教授，從事蛋白質晶體學方麵的研究。2002年獲有機所化學博士學位。在2003-2012年間先後以Postdoctoral Fellow，Research Associate 和Research Scientist 身份在美國國立衛生院(NIH)下屬的癌症研究中心(NCI)，美國紐約紀念斯隆凱特琳癌症中心及美國佐治亞州立大學進行研究。2006年榮獲USA The SER-CAT Young Investigator Award （the Southeast Regional Collaborative Access Team，SER-CAT)；2007年獲得USA FARE Award (The Fellows Award for Research Excellence, NIH)； 2010-2011年獲得USA STTR Grant基金資助。2012年9月受聘万博英超狼队网官方网 研究員。
授課情況
生物化學榮譽課程
招生專業
生物化學
代表性論文和論著
1. Y. Gao, H. Liu, C. Zhang, S. Su, Y. Chen, X. Chen, Y. Li, Z. Shao, Y. Zhang, Q. Shao, J. Li, Z. Huang, J. Ma, J. Gan*, “Structural basis for guide RNA trimming by RNase D ribonuclease in Trypanosoma brucei”, Nucleic Acids Res. Nucleic Acids Res. 49(1): 568-583 (2021)
2. X. Chen, S. Su, Y. Chen, Y. Gao, Y. Li, Z. Shao, Y. Zhang, Q. Shao, H. Liu, J. Li, J. Ma, J. Gan*, “Structural studies reveal a ring-shaped architecture of deep-sea vent”, Nucleic Acids Res. 48(6): 3343-3355 (2020)
3. Y. Chen#, X. Chen#, Q. Huang, Z. Shao, Y. Gao, Y. Li, C. Yang, H. Liu, J. Li, Q. Wang, J. Ma, Y. Zhang*, Y, Gu*, J. Gan*, “A unique DNA-binding mode of African Swine fever virus AP endonuclease”, Cell Discovery, 6(13), doi:10.1038/s41421-020-0146-2 (2020)
4. Y. Zhang, H. Zhang, X. Xu, Y. Wang, W. Chen, Y. Wang, Z. Wu, N. Tang, Y. Wang, S. Zhao, J. Gan*, Q. Ji*, “Catalytic-state structure and engineering of Streptococcus thermophiles Cas9”, Nature Catalysis, doi: 10.1038/s41929-020-00506-9 (2020)
5. B. Wang#, Q. Luo#,Y. Li, L. Yin, N. Zhou, X Li, J. Gan*, A. Dong*, “Structural insights into target DNA recognition by R2R3-MYB transcription factors” Nucleic Acids Res. 48 (1): 460-471 (2020)
6. Y. Chen, H. Liu, C. Yang, Y. Gao, X. Yu, X. Chen, R. Cui, L. Zheng, S. Li, X. Li, J. Ma, Z. Huang*, J. Li*, J. Gan*, “Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues”, Nature Communications. 10(1):387. doi: 10.1038/s41467-019-08296-w (2019)
7. H. Qian, H. Yu, P. Li, E. Zhu, Q. Yao, C. Tai, Z. Deng, K. Gerdes, X. He*, J. Gan*, H. Ou*, “Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex”, Nucleic Acids Res. 47(14), 7690-7702 (2019)
8. W. Chen, H. Zhang, Y. Zhang, Y. Wang, J. Gan*, Q. Ji*, “Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease” PloS Biology 17(10):e3000496 (2019)
9. H. Liu, R. Wang, X. Yu, F. Shen, W. Lan, P. Haruehanroengra, Q. Yao, J. Zhang, Y. Chen, S. Li, B. Wu, L. Zheng, J. Ma, J. Lin, C. Cao*, J. Li*, J. Sheng*, J. Gan*, “High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads”, Nucleic Acids Res. 46(21), 11627-11638 (2018)
10. C. Yang, R. Wu, H. Liu, Y. Chen, Y. Gao, X. Chen, Y. Li, J. Ma, J. Li*, J. Gan*, “Structural insights into DNA degradation by human mitochondrial nuclease MGME1”, Nucleic Acids Res. 46(20), 11075-11088 (2018)
11. H. Liu#, X. Yu#, Y. Chen, J. Zhang, B. Wu, L. Zheng, P. Haruehanroengra, R. Wang, S. Li, J. Lin, J. Li, J. Sheng, Z. Huang*, J. Ma*, J. Gan*, “Crystal structure of an RNA-cleaving DNAzyme”, Nature Communications. 8(1), doi: 10.1038/s41467-017-02203-x (2017)
12. H. Liu, F. Shen, P. Haruehanroengra, Q. Yao, Y. Cheng, Y. Chen, C. Yang, J. Zhang, B. Wu, Q. Luo, R. Cui, J. Li, J. Ma*, J. Sheng*, J. Gan*, “A DNA structure containing AgI-mediated G:G and C:C base pairs”, Angew Chem Int Ed Engl. 56(32):9430-9434 (2017)
13. H. Liu#, C. Chen#, P. Haruehanroengra, Q. Yao, Y. Chen, C. Yang, Q. Luo, B. Wu, J. Li, J. Ma*, J. Sheng*, J. Gan*, “Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex”, Nucleic Acids Res, 45(5):2910-2918 (2017)
14. F. Shen, Z. Luo, H. Liu, R. Wang, S. Zhang, J. Gan*, J. Sheng*, “Structural insights into RNA duplexes with multipile 2’-5’-linkages”, Nucleic Acids Res. 45(6):3537-3546 (2017)
15. Y. Chen, J. Zhang, H. Liu, Y. Gao, X. Li, L. Zheng, R. Cui, Q. Yao, L. Rong, J. Li, Z. Huang*, J. Ma*, J. Gan*, “Unique 5’-P recognition and basis for dG:dGTP misincorporation of ASFV DNA polymerase X”, PloS Biology 15(2):e1002599 (2017)
16. J. Zhang, H. Liu, Q. Yao, X. Yu, Y. Chen, R. Cui, B. Wu, L. Zheng, J. Zuo, Z. Huang*, J. Ma*, J. Gan*, “Structural basis for single-stranded RNA recognition and cleavage by C3PO”, Nucleic Acids Res, 44(19):9494-9504 (2016)
17. J. Zhao#, X. Yu#, M. Zhu, H. Kang, J. Ma, M. Wu, J. Gan*, X. Deng*, H. Liang*, Structural and molecular mechanism of CdpR involved in quorum-sensing and bacterial virulence in Pseudomonas aeruginosa, PloS Biology, 14(4):e1002449 (2016).
18. J. Gan, J. Tropea, B. Austin, D. Court, D. Waugh, X. Ji*, “Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III” Cell, 124(2), 355–366 (2006)